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infections among diarrheal outpatients in Shanghai: a retrospective case study
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《医学前沿(英文)》 2018年 第12卷 第1期 页码 98-103 doi: 10.1007/s11684-018-0614-3
Cyclospora cayetanensis is a foodborne and waterborne pathogen that causes endemic and epidemic human diarrhea worldwide. A few epidemiological studies regarding C. cayetanensis infections in China have been conducted. During 2013, a total of 291 stool specimens were collected from patients with diarrhea at a hospital in urban Shanghai. C. cayetanensis was not detected in any of the stool specimens by traditional microscopy, whereas five stool specimens (1.72%, 5/291) were positive by PCR. These positive cases confirmed by molecular technology were all in the adult group (mean age 27.8 years; 2.94%, 5/170) with watery diarrhea. Marked infection occurred in the rainy season of May and July. Sequence and phylogenetic analyses of the partial 18S rRNA genes of C. cayetanensis isolated showed intra-species diversity of this parasite. This study showed, for the first time, that C. cayetanensis is a pathogen in outpatients with diarrhea in Shanghai, albeit at a low level. However, the transmission dynamics of this parasite in these patients remain uncertain.
关键词: Cyclospora cayetanensis outpatients with diarrhea stool specimens 18S rRNA gene
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《医学前沿(英文)》 2018年 第12卷 第2期 页码 196-205 doi: 10.1007/s11684-017-0560-5
We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridium difficile genes, including tpi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC for simultaneous detection and characterization of toxigenic C. difficile directly from fecal specimens. The mPCR-CE had a limit of detection of 10 colony-forming units per reaction with no cross-reactions with other related bacterial genes. Clinical validation was performed on 354 consecutively collected stool specimens from patients with suspected C. difficile infection and 45 isolates. The results were compared with a reference standard combined with BD MAX Cdiff, real-time cell analysis assay (RTCA), and mPCR-CE. The toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens by the mPCR-CE, which provided a positive rate of 20.3% (81/399). The mPCR-CE had a specificity of 97.2% and a sensitivity of 96.0%, which was higher than RTCA (x2 = 5.67, P = 0.017) but lower than BD MAX Cdiff (P = 0.245). Among the 45 strains, 44 (97.8%) were determined as non-ribotype 027 by the mPCR-CE, which was fully agreed with PCR ribotyping. Even though ribotypes 017 (n = 8, 17.8%), 001 (n = 6, 13.3%), and 012 (n = 7, 15.6%) were predominant in this region, ribotype 027 was an important genotype monitored routinely. The mPCR-CE provided an alternative diagnosis tool for the simultaneous detection of toxigenic C. difficile in stool and potentially differentiated between RT027 and non-RT027.
关键词: Clostridium difficile multiplex PCR capillary electrophoresis detection characterization
Analysis of stress and failure in rock specimens with closed and open flaws on the surface
《结构与土木工程前沿(英文)》 2021年 第15卷 第5期 页码 1222-1237 doi: 10.1007/s11709-021-0773-1
关键词: surface flaw heterogeneity circular hole numerical modeling relative displacement
Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell
Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo
《医学前沿(英文)》 2019年 第13卷 第4期 页码 492-503 doi: 10.1007/s11684-019-0681-0
关键词: haploidentical hematopoietic stem cell transplantation stool culture Candida
Baoyun ZHAO, Yang LIU, Dongyan LIU, Wei HUANG, Xiaoping WANG, Guibao YU, Shu LIU
《结构与土木工程前沿(英文)》 2020年 第14卷 第2期 页码 322-330 doi: 10.1007/s11709-019-0594-7
关键词: rock-concrete composite specimen contact surface mechanical properties failure mechanism
《结构与土木工程前沿(英文)》 2021年 第15卷 第6期 页码 1372-1389 doi: 10.1007/s11709-021-0772-2
关键词: soft−hard composite layered rock mass double cracks crack evolution acoustic emission digital image correlation
WAN Zhen-ping, LIU Ya-jun, TANG Yong, YE Bang-yan
《机械工程前沿(英文)》 2006年 第1卷 第2期 页码 194-198 doi: 10.1007/s11465-005-0011-x
关键词: different 30o-120o relationship initiation cutting parameter
Numerical evaluation of group-pile foundation subjected to cyclic horizontal load
Youngji JIN, Xiaohua BAO, Yoshimitsu KONDO, Feng ZHANG,
《结构与土木工程前沿(英文)》 2010年 第4卷 第2期 页码 196-207 doi: 10.1007/s11709-010-0021-6
关键词: group-pile foundation real-scale cyclic loading test three-dimensional finite element method (3D-FEM) soil-water coupling analysis undisturbed and remolded specimens
吴智敏,徐世烺,丁一宁,卢喜经,刘佳毅,丁生根
《中国工程科学》 2001年 第3卷 第4期 页码 76-81
采用跨高比为2.5的非标准三点弯曲梁试件,利用在试验中测得的最大荷载Pmax及对应的裂缝口张开位移CMODc,根据渐近线性叠加假设,求得了砼裂缝亚临界扩展量∆ac。在此基础上,采用虚拟裂缝模型计算了不同初始缝长、不同试件髙度的砼非标准三点弯曲梁试件的起裂断裂韧度KiniIC、失稳断裂韧度KunIC及临界裂 缝尖端张开位移CTODc。计算结果表明,砼KunIC:与试件高度及初始缝高比无关;而KiniIC在初始缝髙比a0/h = 0.3〜0.5内,也是一稳定的常数且与试件高度无关。这说明砼双K断裂参数可以作为砼的材料常数。
标题 作者 时间 类型 操作
Simultaneous detection and characterization of toxigenic:Clostridium difficile directly from clinical stoolspecimens
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期刊论文
Positive stool culture could predict the clinical outcomes of haploidentical hematopoietic stem cell
Lijuan Hu, Qi Wang, Xiaohui Zhang, Lanping Xu, Yu Wang, Chenhua Yan, Huan Chen, Yuhong Chen, Kaiyan Liu, Hui Wang, Xiaojun Huang, Xiaodong Mo
期刊论文
on the influence of contact surface constraint on mechanical properties of rock-concrete composite specimens
Baoyun ZHAO, Yang LIU, Dongyan LIU, Wei HUANG, Xiaoping WANG, Guibao YU, Shu LIU
期刊论文
Crack evolution of soft–hard composite layered rock-like specimens with two fissures under uniaxial compression
期刊论文
Initiation and propagation laws of the glass cracks in specimens subjected to normal loading under the
WAN Zhen-ping, LIU Ya-jun, TANG Yong, YE Bang-yan
期刊论文
Numerical evaluation of group-pile foundation subjected to cyclic horizontal load
Youngji JIN, Xiaohua BAO, Yoshimitsu KONDO, Feng ZHANG,
期刊论文